|Enemies||Xerxes I of Persia, Artemisia|
|First seen||300: Rise of an Empire|
|Last seen||300: Rise of an Empire|
Calisto is one of the main characters in 300: Rise of an Empire.
300: Rise Of An Empire
Themistocles leads his men, which include Scyllas, Scyllas' son Calisto and Themistocles' right-hand man Aesyklos to the Aegean Sea. They ram their ships into the Persian ships, charge them, slaughtering several soldiers before retreating from the sinking Persian ships. The following day, the Greeks feign a retreat and lead a group of Persian ships into a crevice, where they become stuck. The Greeks charge the Persian ships from the cliffs above and kill more Persians. Impressed with Themistocles' skills, Artemisia brings him onto her ship where she attempts to use sex to seduce him to join the Persians as her second-in-command. He refuses, causing her to push him aside and swear revenge.
The Persians spill tar into the sea and send suicide bombers to swim to and board the Greek ships with their flame bombs. Artemisia and her men fire flaming arrows and torches to ignite the tar, but Themistocles manages to kill one of the soldiers, who falls into the tar carrying a torch, causing ships from both sides to explode. Themistocles is thrown into the sea by an explosion and nearly drowns before being rescued by Calisto, and stands by Scyllas' side as he succumbs to his injuries. Believing Themistocles to be dead, Artemisia and her forces withdraw.